Carolyn S. Glaubensklee, Ph. D.

 

Education               Ph.D., Medical Science, Physiology, 1985, University of New Mexico School of                                 Medicine,

 

                                Dissertation title: Involvement of Calcium and Calmodulin in the 

                                Regulation of Water Permeability and Anion Transport across the Erythrocyte

                                Membrane: APossible Defect in Cystic Fibrosis.

 

                                M.S., Medical Science, Physiology, 1979, University of New Mexico School of

                                Medicine.   

                               

                                Thesis Title: Membrane Protein Analysis of Human erythrocytes of the McLeod                                 

                                Phenotype Utilizing Discontinuous Polyacrylamide Gel Electrophoresis and Freeze                                

                                Fracture Electron Microscopy.

 

                                B.S., Biology/Chemistry Double Major, 1975, with distinction, University of

                                Southern Colorado.

 

Employment          Colorado State University-Pueblo/Department of Nursing/Assistant Professor /2004-present

 

Colorado State University-Pueblo/Department of Biology/Visiting Assistant Professor/1999-2004.

 

The Colorado College/Department of Biology/Assistant Professor/1992-1994/Adjunct Assistant  Professor /1994-Present / Pre-Health Advisor/1994-1999/Adjunct Assistant Professor Emeritus/1999

 

                                Pikes  Peak Community College/Lecturer/ Anatomy & Physiology/1990-1992

 

                                UC Berkeley/Physiology-Anatomy/Lecturer/Post Doctoral Associate 1987-1989

 

                                UCSF/Cardiovascular Research Institute/Cystic Fibrosis Foundation Fellow 1985-86

 

                                Post-doctoral position (CVRI) Training Grant 1986-87

 

University of New Mexico School of Medicine 1976-1979. Research assistant working on M.S. degree. Position included working on research labs and lecture teaching responsibilities.

 

                                USC Biology Department, B.S. Biology and Chemistry, Pueblo, CO. 9/73-9/74.

                                Student research assistant preparation for physiology laboratories.

 

                                Colorado Fuel and Iron Corp. Chemistry Lab., Pueblo, CO. 4/76-8/76

 

Teaching                Principles of Biology-Introduction to Cell Biology/The Human Breast/     

Experience             Mammology/Vertebrate Physiology/Human Anatomy & Physiology: USC, 1999-present

 

Vertebrate Physiology-Animal Cell Physiology-Intro to Pharmacology-Cell Biology and Vertebrate Zoology: Colorado College 1992 to present

 

Bethel College of Nursing, Adjunct Assistant Professor:  Guest Lecturer on Cancer & Neoplastic  Drugs

 

                                Pikes Peak Community College, Lecturer, Cadaver Anatomy & Physiology, 1990- 

                                1992

 

                                Physiology 1-Lectures, Fall Semester: UC-Berkeley Physiology Dept. 1988.

 

                                Physiology 1-Summer Session: UC-Berkeley Physiology Dept. 1988. (Lecture and

                                Lab.).

 

                                Cell Permeability Labs for Medical Students: Teaching Assistant. UNM Medical

                                school.

                                (1979-1981).

 

Nerve and muscle Instrumentation labs for medical students: Teaching Assistant at UNM Medical School. (1979-1981).

 

                                Cardiac Dynamics Lab for medical students: Teaching assistant, UNM (1977-1982).

 

                                General Physiology Lectures to Physical Therapists: Lecturer (1983).

 

                                GI Lectures to medical students at UNM: Lecturer (1983).

 

                                Basic Science Enrichment Lectures: Lecturer (1983).

 

Invited

Lectures                 I was an invited speaker at the International Cystic Fibrosis Congress in Brighton, England, where I presented my dissertation work.  This trip was fully funded by the Congress.

 

                                Poster presentation to Trieste, Italy concerning Ph.D. work.

 

Poster presentation in Vancouver, British Columbia, as part of the American Physiological Society presenting SPQ  research as a chloride sensitive probe in cystic fibrosis research.

 

 

Research                I have participated in cystic fibrosis research investigating membrane dynamics for

Experience             approximately seven years. My graduate research was on membrane transport across                                 erythrocyte membranes and the regulation of the process by calcium and calmodulin. I                                 have worked in membrane biophysics using fluorescent dyes as voltage-sensitive

                                probes to investigate water transport and chloride conductance in the human placenta 

                                of normal and cystic fibrosis tissues. I have become knowledgeable in several 

                                laboratory technique including electophoretic protein separation, transmission and 

                                freeze-fracture EM. Recent experience includes use of the Dionex stopped-flow          

                                spectophotometer for determination of hydraulic water permeability, atomic

                                absorption for calcium analysis, RIA for detection of calmodulin and have worked on

                                fluorescent probes utilizing a SLM 480 Fluorimeter interfaced to an IBM PC/XT

                                computer. I have been involved in using electron paramagnetic resonance to

                                investigate conformational change in the calmodulin molecule with titration of calcium.

                                I have also been involved ininvestigation calcium/calmodulin abnormalities as they

                                relate to sickle cell disease.

 

Academic               Member of the Association of Clinical Anatomists (Sponsored Membership)-1997

Honors &

Fellowships           Member of the National Association of Health Professions Advisors:1994-Present

               

Advisory panel member for the Betz Newsline-A Newsletter for Pre-health Advisors-1996-97.

 

                                Cystic fibrosis Fellow, UCSR/CVRI, Fall 1985.

                               

                                Post-Doctoral Position (2nd Year) Training Grant UCSF/CVRI: 1986-1987.

 

                                Strobel Research Grant (ALA) Recipient 1987-1988.

 

                                Biophysical Society 1987.

 

                                Sigma X membership 1985-current

 

                                Cystic Fibrosis Student Traineeship;. Summer, 1980.

 

                                UNM Graduate Student Fellowship, Fall, 1979.

 

                               

Community College

Service,

Activities &

Committees           Health Professions Advisor-1994-1999/Colorado College

                               

                                Faculty Sponsor for the Pre-Health Club on campus--1995-1999/Colorado College

                               

                                Member of the Neuroscience Advisory Board/Colorado College

 

                                Member of the Chem-links Advisory Board/Colorado College

 

                                Member of the Human Subject Review Committee for Colorado College

 

                                Invited speaker for the College Relations  Speaker’s Bureau

 

                                Faculty Sponsor for the “Run for Smiles”  Benefit run for Operation Smile-1995-1999

 

Courses

Taught at CC

USC                        Vertebrate Zoology                                                 Introduction to Pharmacology

 

                                Introduction to Holistic Medicine &                      Human Physiology

                                Natural Homeopathy

 

                                Cell Biology                                                            Principles of Biology

 

                                Animal Cell Physiology                            

                                                                                                          

                                Mammalogy                                                           

 

Vertebrate Physiology                                            Human Breast Anatomy &

                                                                                Physiology

 

                                Human Anatomy & Physiology                             Introduction to Cell Biology

 

 

New Courses         Helped to propose & develop the plans for the new cadaver anatomy & physiology course/CC.

 

Helped to propose & develop the Wilderness Medicine course taught by Dr. Peter Hudson over the half-block/CC

 

                                Helped to propose & develop the Introduction to Pharmacology course/CC

 

Helped to propose & develop the Introduction to Holistic Medicine & Natural Homeopathy course/CC

 

 

Internship

Programs               Rural Health Internship Program -1994-Present

Developed              Involves serveral rural doctors who have volunteered their time & services to

                                                engage our students

 

                                Inner City Internship Program-Tentative Start Date Winter 1997

                                                Involves shadowing, volunteer experience, surgical observation

                                                and medical research opportunities in collaboration with

                                                Penrose Hospital

 

                                International Medical Internship Program--1997-Present

                                                Student exchange program involving CC

                                                students and students from the UAG in Guadalajara, Mexico and with

                                                St. George’s Medical School in Granada, West Indies.

 

Research

Students

Gretchen Greer--”How Women Respond to Cold:  The Physiology of Prrimary Raynaud’s Phenomenon”--1994:Winner of Presentation Award at the Undergraduate Research Conference in San Diego, CA. Graduated with Distinction.

 

                                Eric Kuhn--”Isolation of Norepinephrine and Dopamine Receptor Sites in the Medial

                                Medulla of the Rat Brain Utilizing Tyrosine Hydroxylase Immunocytochemistry”--

                                1995-Presented at the Undergraduate Research Conference held in San Francisco,

                                CA.:

                                Graduated with Distinction.

 

                                Spencer Bonnie--p53 Research in Lung Cancer--Library project.

 

                                Chris Erb--”An evaluation of a National Pediatric Health Care Program in Costa Rica:

                                The fullfillment of the goals of the Carne de salud del nino.”--1997-Graduated with

                                Distinction. 

 

                                Crystal Taylor--p53 Research in Cancer: Summer 1997-1999.

 

 

Publications          C.S. Glaubensklee, A.P. Evan and W.R. Galey: Structural and biochemical analysis of                                        the McLeod erythrocyte membrane. Vox Sang. 42:262, 1982.

 

                                C.S. Glaubensklee, and W.R. Galey: Decreased erythrocyte water permeability in                                 cystic fibrosis: Investigation of the role of calcium and calmodulin. In: Calcium-

                                Binding Proteins. Ec. B. deBernard, et al., Elsevier publishers, New York, NY., 1983.

 

                                C.S. Glaubensklee, and W.R. Galey: Calcium and calmodulin are involved in the                                 decreased water permeability of cell membranes in cystic fibrosis, Proc. 9th                                 International Congress on Cystic Fibrosis, Cystic Fiborsis Horizons J. Wiley & 

                                Sons, D. Lawson Editor, 1984 NY, NY.

 

                                C.S. Glaubensklee, A.P. Evan and W.R. Galey: Further characterization of the

                                McLeod erythrocyte, Fed. Proc. 38:1128, 1979.

 

                                C.S. Glaubensklee, and W.R. Galey: Erythrocyte osmotic water permeability:                                 investigations on the role of calcium and calmodulin. Fed. Proc. 42(4):

 

                                C.S. Glaubensklee, M.A. Zanner and W.R. Galey: Regulation of anion transport

                                across the erythrocyte of normal and cystic fibrosis individuals. Fed. Proc. 43(4):

                               4639,1984.

 

                                C.S. Glaubensklee, and W.R. Galey: Decreased erythrocyte water permeability in                                 cystic fibrosis: Investigation of the role of calcium and calmodulin. Cystic Fibrosis                                 Club Abstracts, Vol. 24, Pg. 113, 1983.

 

                                N. Ilsley, C.S. Glaubensklee, B. Davis and A.S. Verkman: Chloride transport across                                 placental microvillous membranes measured by a chloride-sensitive flourescent probe.                                 Am. J. of Phy. 255 (Cell Physiol 24) C 789-797, 1988.

 

                                C.S. Glaubensklee, N. Ilsley, B. Davis and A.S. Verkman: Cystic Fibrosis Research                                  Center, CVRI: Chloride pathway in human placental microvillus membrane vesicles.                                  Biophysical Journal, Feb., 1987. Vol 51 No. 2 part 2 page. 344a.

 

                                B. Davis, J. Goodman, N. Ilsley, C.S. Glaubensklee, and A.S. Verkman, UCSF: ATP

                                and catalytic subunit of protein kinase A stimulate chloride transport in placental                                 microvillus membrane vesicles. North American CF Conference Toronto, Ontario,                                 Canada, Fall 1987. Journal of Pediatric Pulmonology.

                               

ACTIVE

GRANTS:             

                                P20-NIH Grant written in conjunction with the University of Colorado Health 

                                Sciences Center—Funded Sept 16th 2002/2005.